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. 2020 Nov 18;124(1):290–298. doi: 10.1038/s41416-020-01146-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Relative cell growth (%) of BEAS-2B cells expressing empty vector (Vector) or CD63–BCAR4. Cell proliferation of BEAS-2B (bronchial epithelial cell line) and NCI-H1299 (lung cancer cell line) was measured using the IncuCyte™ Live-Cell Imaging System. b Representative colonies and relative colony-forming activity of CD63–BCAR4 or empty vector-expressing cells. c Representative DNA histograms and bar graph of cell-cycle distribution at the indicated time points after a double-thymidine block. Overexpressed BEAS-2B cells were synchronised in G1 by a double-thymidine block, and then released into the cell cycle for the times indicated. Each bar in the graph indicates the average cell-cycle distribution from triplicated experiments. d Expression levels of cell-cycle-related genes after exogenous expression of CD63–BCAR4 and BCAR4. The relative expression was compared to the control gene GAPDH by using semi-quantitative RT-PCR. Data in this figure are presented as the representative results of three independent experiments. Bar graphs demonstrated the mean ± SD. Statistically significant differences are marked with *(p < 0.05), ** (p < 0.005) and ***(p < 0.0005), respectively.