Figure 2.

Overexpression of dusp1 mimics the DNFR function. (A) Dusp1 mRNA (3 ng/embryo) was injected at the 4 cell stage in dorsal half and harvested at stage 15 to identify the morphological change as compared to that of control (uninjected whole embryos). (B) Dusp1 (3 ng/embryo) was injected and harvested at stage 11-11.5. Immunoprecipitation was performed with pJnk, pErk, pSmad1C (c-terminal), pSmad1L (Linker region), pSmad2C (c-terminal) and α-tubulin was included as a control (α-tubulin being a housekeeping protein).