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. 2020 Nov 27;24(6):359–370. doi: 10.1080/19768354.2020.1847732

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Dusp1 regulates reporter activities of ARE, BRE and SCL promoter constructs. (A and B) The reporter ARE construct (40 pg/embryo) was injected with or without dusp1 (3 ng/embryo) at the one-cell stage and the embryos were grown until stage 11 and 18 for the relative reporter activity. (C) The reporter BRE construct (40 pg/embryo) was injected with or without dusp1 (3 ng/embryo) and/or fgf8b (1 ng/embryo) or together (with or without treatment with BCI and U0126) and the embryos at stage 11 to measure the relative reporter activity. (D) SCL promoter (40 pg/embryo) was injected with or without dusp1 (3 ng/embryo) at stage 11 to measure the relative promoter activity. The data are shown as mean ± S.E. of the values from at least three independent experiments. Differences were considered significant at P < 0.05.