Table 2.
Anion inhibition data of BteCAι as determined by a stopped-flow CO2 hydrase assay.20
| Anion | KI (mM)a |
|||
|---|---|---|---|---|
| hCA Ib | hCA IIb | E. coli β-CAc | BteCAιd | |
| F− | >300 | >300 | 9.4 | 4.6 |
| Cl− | 6 | 200 | 6.7 | 3.1 |
| Br− | 4 | 63 | 3.8 | 4.8 |
| I− | 0.3 | 26 | >50 | >50 |
| CNO− | 0.0007 | 0.03 | 0.58 | 0.79 |
| SCN− | 0.2 | 1.6 | 5.7 | 6.1 |
| CN− | 0.0005 | 0.02 | >50 | >50 |
| N3− | 0.0012 | 1.51 | >50 | >50 |
| NO2− | 8.4 | 63 | 4.9 | 8.4 |
| NO3− | 7 | 35 | 2.4 | 6.2 |
| HCO3− | 12 | 85 | 0.81 | 0.94 |
| CO32− | 15 | 73 | 0.89 | 4.4 |
| HSO3− | 18 | 89 | 3.7 | 8.4 |
| SO42− | 63 | >200 | 1.7 | 5.8 |
| HS− | 0.0006 | 0.04 | 2.7 | 6.2 |
| NH2SO2NH2 | 0.31 | 1.13 | 0.011 | 0.086 |
| NH2SO3H | 0.021 | 0.39 | 0.0025 | 0.0062 |
| PhAsO3H2 | 31.7 | 49 | 0.0061 | 0.008 |
| PhB(OH)2 | 58.6 | 23 | 0.0028 | 0.009 |
| ClO4− | >200 | >200 | >50 | >50 |
| SnO32− | 0.57 | 0.83 | 0.52 | 0.094 |
| SeO42− | 118 | 112 | 3.1 | 0.73 |
| TeO42− | 0.66 | 0.92 | 0.51 | 0,71 |
| OsO52− | 0.92 | 0.95 | >50 | >50 |
| P2O72− | 25.8 | 48 | >50 | >50 |
| V2O72− | 0.54 | 0.57 | >50 | >50 |
| B4O72− | 0.64 | 0.95 | 0.25 | >50 |
| ReO4− | 0.11 | 0.75 | >50 | >50 |
| RuO4− | 0.101 | 0.69 | 9.5 | >50 |
| S2O82− | 0.107 | 0.084 | 6.4 | 7.4 |
| SeCN− | 0.085 | 0.086 | 3.1 | 6.6 |
| NH(SO3)22− | 0.31 | 0.76 | 1.5 | >50 |
| FSO3− | 0.79 | 0.46 | 0.83 | 9.3 |
| CS32− | 0.0087 | 0.0088 | 3.1 | 8.6 |
| EtNCS2− | 0.00079 | 0.0031 | 0.084 | 0.81 |
| PF6− | >50 | >50 | >50 | >50 |
| CF3SO3− | >50 | >50 | >50 | >50 |