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. 2020 Dec 31;13:13357–13370. doi: 10.2147/OTT.S281519

Figure 3.

Figure 3

Effect of VCX treatment on the activity and expression of pro- and anit-apoptotic markers in MDA-MB-231cells. MDA-MB-231 cells were treated for 24 h with VCX (10, 25, and 50 μM). (A) The percentage of cells expressing caspases 3/7 was determined using Muse® Caspases 3/7 Kit. The histogram represented the mean, (n = 3, triplicate). *P < 0.05 compared to control, VCX=0 μM, (ANOVA followed by SNK test). (B) Caspases 3 and 7 mRNA levels were quantified using qRT-PCR and normalized to β-actin housekeeping gene. Triplicate reactions were performed for each experiment. The values represented the mean of fold change ± SEM, (n = 6). *P < 0.05 compared to control, VCX=0 μM, (ANOVA followed by SNK tst). (C) caspase 3, BAX and BCL-2 protein levels were determined by Western blot analysis. One of three representative experiments is shown. The values represented the mean of fold change ± SEM, (n = 3). *P < 0.05 compared to control, VCX= 0 μM, (ANOVA followed by SNK test). (D) Histogram represents BAX/BCL-2 ratio.