Figure 6.

Effect of VCX treatment on autophagy in MDA-MB-231cells. MDA-MB-231 cells were treated for 24 h with VCX (25 μM). (A) LC3 and Beclin1 mRNA levels were quantified using qRT-PCR and normalized to β-actin housekeeping gene. The values are presented as mean ± SEM, (n = 6, triplicate). *P < 0.05 compared to control, VCX=0 μM, (Student’s t-test). (B) LC3I and II protein levels were determined by Western blot analysis. One of three representative experiments is shown. The values are presented as mean ± SEM. *p< 0.05 compared to control, VCX=0 μM, (Student’s t-test).