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. 2020 Dec 22;8:610587. doi: 10.3389/fcell.2020.610587

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Copyright © 2020 Zhang, Wang, Yang, Qiao, Li, Guo, Ding, Li, Ye, Wu, Cui and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of exosomes and the uptake of exosomes from USCs by HK-2 cells. (A) Morphology of USC-Exos under TEM (scale bar = 200 nm). (B) The expression of CD63, TSG101, and ALIX in exosomes, MPs and USCs was determined by Western blot analysis. (C) The mean diameter and the concentration of exosomes were measured by Nanoparticle tracking analysis. (D) The mean diameter and the concentration of MPs were measured by Nanoparticle tracking analysis. (E) Green fluorescent protein (GFP) was introduced into HK-2 cells, exosomes labeled with red fluorescent dye Dil are shown to be up take by HK-2 cells.