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. 2020 Dec 22;8:610587. doi: 10.3389/fcell.2020.610587

FIGURE 6.

FIGURE 6

USCs-Exos play a protecting role by regulating the miR-216a-5p/PTEN/AKT signaling pathway. (A) The expression of miR-216a-5p in HK-2 cells treated with the miR-216a-5p mimic and inhibitor by real time PCR. *P < 0.05 versus PBS control, n = 3. (B) The expression of miR-216a-5p in HK-2 cells treated with exosomes derived from USCs transfected with the miR-216a-5p mimic and inhibitor. *P < 0.05 versus PBS control, n = 3. (C) Cell proliferation of HK-2 cells measured by CCK8 assay after administration of exosomes derived from USCs transfected with the miR-216a-5p mimic and inhibitor. *P < 0.05 versus PBS control, n = 3. (D) Expression of PTEN and phosphorylation of Akt by Western blot. (E) Graph depicts caspase-3 activity in HK-2 cells exposed to H/R, with or without transfection with siRNA to PTEN, and with or without USCs-Exos.*P < 0.05 versus all other groups; n = 3. (F) Representative blots for PTEN and Actin are depicted. Five microliters of mimic and anti-miR with concentration of 20 pmol/μl were added, respectively. *P < 0.05 versus normoxic conditions, n = 3. (G) Representative blots for phosphorylated Akt and total Akt are depicted. *P < 0.05 versus normoxic conditions, n = 3.