Figure 4. Effect of astaxanthin on the expression of c-myc and cyclin D1 in the gastric mucosal tissues of H. pylori-infected mice.
(A, B) c-myc and cyclin D1 mRNA levels in the gastric mucosal tissues as assessed by RT-PCR; GAPDH mRNA was used to normalize the results. (C, D) Upper: Western blot analysis of c-myc and cyclin D1. Lower: graph showing the levels of each protein as a density ratio relative to the density of the loading control (β-actin). The density ratio of the None group was set as 100%. None, non-infected mice fed standard chow; Control (H. pylori control), H. pylori-infected mice fed standard chow; ASX (H. pylori + ASX), H. pylori-infected mice fed chow supplemented with astaxanthin (ASX). aP < 0.05 vs. non-infected controls (None), bP < 0.05 vs. infected controls (H. pylori control).