Table 1.
Gene | Primer | Sequence (5’-3’) |
---|---|---|
GAPDH | Probe | FAM-TCATACTTCTCATGGTTCACACCCATCACG-BHQ1 |
Forward | TTGCTGACAATCTTGAGGGAGTT | |
Reverse | CTGCTGATGCCCCCATGT | |
IFN-γ | Probe | FAM-CTCACAGACCTCCAGCAT-BHQ1 |
Forward | TGCCATCCATGCGGAAA | |
Reverse | AGCGGGAAGAACTCCTCTTC | |
c-myc | Probe | FAM-ACCTCCGCACAGCCCACTGGT-BHQ1 |
Forward | AGCGACTCTGAAGAAGAGCA | |
Reverse | CGTAGTTGTGCTGGTGAGTG | |
Cyclin D1 | Probe | FAM-TCACCATCCTTTTGCCAGTTCCTCCAG-BHQ1 |
Forward | TCAAGTGGCATAGATGTGGAAGAA | |
Reverse | TGGCTCTGCAGGATTTTCATG |
RT-PCR, real-time PCR.