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. 2020 Dec 22;8:615264. doi: 10.3389/fcell.2020.615264

FIGURE 6.

FIGURE 6

Disruption of CXCR4 signaling in the early head mesoderm and BA2 inhibits migration of the BA2-mesodermal progenitor cells. (aA) Schematic representation of chicken embryo at HH11 implanted with beads soaked with the CXCR4 inhibitor AMD3100 or with PBS in head mesoderm. The embryos were re-incubated until they reached stages HH20 and hybridized with a Tbx1 probe. (aB,aC) Schematic representation showing the head region and the location of mesodermal cells in the BA2 at HH20. Tbx1 expressing area in the BA2 (black arrow) was diminished in AMD3100-treated embryos (B) in comparison with the PBS-treated embryos (C). (bA) Schematic representation of embryo at HH16 implanted with beads soaked with the AMD3100 or with SDF-1 in head mesoderm. PBS bead used as control. Myf5−expressing region (blue arrow in bC) was increased in SDF-1-treated embryo. The BA2 myogenic core region (black arrow in bD) was reduced in AMD3100-treated embryos, but not in PBS-treated embryos (bB). Green arrows show the location of the implanted beads.