The nitrate reduction of wild-type strain of S. loihica PV-4, the in-frame deletion mutants Δcrp1, Δcrp2, and ΔnirK, and the Δcrp1 ΔnirK double mutant under high carbon/nitrogen ratios. The concentrations of NO3− (a), NO2− (b), NH4+ (c), NO (d), and N2O (e) in the medium were measured under high carbon/nitrogen ratios (C/N = 12). Lactate (10 mM) and nitrate (2 mM) were added to phosphate-buffered basal salt medium supplemented with 0.5 mM ammonium chloride as a nitrogen source.