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Electrophoretic mobility shift assay (EMSA) analysis on CRP1 binding to the promoter sequence of nap-α, nap-β, nrfA, and nirK. No binding of CRP1 with the nirK promoter sequence was observed in the presence of increasing levels of cyclic AMP. There was still binding observed in the nap-α promoter region, although there is no typical CRP1-recognized motif.
