FIG 6.

(a) Quantitative PCR analyses on transcriptional profile of CRP/FNR homologues in the wild-type strain S. loihica PV-4 under high carbon/nitrogen ratios and low carbon/nitrogen ratios. H, C/N = 6; L, C/N = 2. (b) Quantitative PCR analyses of transcriptional profile of nrfA, crp2, and nirK in the wild-type strain S. loihica PV-4 and in-frame deletion mutant Δcrp under high carbon/nitrogen ratios. Gene expression was normalized against the 16S rRNA gene using the 2^−ΔCT calculation as follows: ΔC_T = C_{Tgene of interest} − C_{T16S rRNA}. Results are the means of three replicates, and error bars indicate standard deviation.