FIG 6.

RcsB contributes to capsular polysaccharide production (CPS) and biofilm formation by S. marcescens. (A) RcsB positively controls CPS production in S. marcescens. (B) RcsB positively regulates biofilm formation by S. marcescens. (C) Gene organization of the putative capsular polysaccharide biosynthesis gene clusters in S. marcescens. Arrows without gene designations represent genes that encode hypothetical proteins. Genes belonging to the same operon are represented by the same color, as indicated. (D) RT-qPCR analysis of the difference in expression of capsular polysaccharide biosynthesis-related genes galU, wza, neuB, wzx, wzy, manC, and manB between strain SK68 and its parent strain JNB5-1. (A, B, and D) Experiments were performed in biological triplicates. Error bars indicate the standard deviations. One-way ANOVA was used to examine the mean differences between the data groups. ****, P < 0.001.