TABLE 2.
Primers used in this study
| Function | Primer | Primer sequence (5′–3′)a |
|---|---|---|
| Identification of Tn5G in mutant SK68 | OTn1 | GATCCTGGAAAACGGGAAAG |
| OTn2 | CCATCTCATCAGAGGGTAGT | |
| Amplification of metR gene for complement expt | RcsB-F1 | CGCGAATTCATGAATAACCTGAACGTAATTATTGCTGATGACC |
| RcsB-R1 | CGCGGATCCTTAGTCTTTGTCCAACGGCGTCATG | |
| Overexpression of metR in plasmid pET28a | RcsB-F2 | CGCGGATCCATGAATAACCTGAACGTAATTATTGCTGATGACC |
| RcsB-R2 | CGCAAGCTTTTAGTCTTTGTCCAACGGCGTCATG | |
| Amplification of the pig operon promoter region | PigA-F1 | CGCGGATCCGTGTTATTTATACACAAAAATTTACATATAATAAAAATCTTAATTTGATC |
| PigA-R1 | CGCGAATTCTTTTTCCTCCGGAATGCTCCTGC | |
| Amplification of the pig operon promoter and operator region | PigA-F2 | CGCGGATCCGACGAACTCCGCCATTGGGTTG |
| PigA-R2 | CGCGAATTCTTTTTCCTCCGGAATGCTCCTGC | |
| Amplification of the pig operon promoter region, operator region, and coding sequences of pigA | PigA-F3 | CGCGGATCCTGCGCCTCCCCGCAGACC |
| PigA-R3 | CGCGAATTCTTTTTCCTCCGGAATGCTCCTGC | |
| Amplification of the flhDC gene promoter region | FlhDC-F | CGCGGATCCATTCCCCATCCCGACAGACTATG |
| FlhDC-R | CGCGAATTCGCTGCTTGCGTTGAAAAACCG |
a
Underlining indicates the added restriction enzyme sites.