FIG 4.

Transcript counts per million reads (CPM) for genes of interest in CH₄ cycling normalized by the TMM method. Reads with homology to transcripts for methyl-coenzyme M reductase (mcrABGCD) and the colocalized tetrahydromethanopterin S-methyltransferase subunits (mtrACDEH), particulate CH₄ monooxygenase (pmoABC), formate dehydrogenase alpha subunit (fdhA), and carbonic anhydrase (CA) are shown. A negative control wherein no reverse transcriptase was added to the PCR (NoRT) had no transcripts for any gene within this subset.