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. 2020 Dec 22;11:606518. doi: 10.3389/fimmu.2020.606518

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Copyright © 2020 Yamazaki, Biswas, Kosugi, Nagashima, Inui, Tomono, Takagi, Ichimonji, Nagaoka, Ainai, Hasegawa, Chiba and Akashi-Takamura

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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αRP105-TM can associate with the B cell membrane, depending on RP105. (A) From Figures 6B, C , the splenocytes were collected and incubated with FITC-conjugated anti-mouse IgG1 and APC-conjugated anti-mouse IgD. (B) From Figures 7A, B , the splenocytes were collected and incubated with FITC-conjugated anti-mouse IgG1 and APC-conjugated anti-mouse IgD. The indicated numbers on each gate in the left panel represents the percentage of cells of IgD⁺mIgG1^- or IgD⁺mIgG1⁺ gated on B220⁺ cells, respectively. The histogram and bar graph in the panels on the right represent the level of mIgG1, which represents the level of αRP105 on B220⁺ IgD⁺cells. The expression level was analyzed using a BD LSRFortessa. All indicated data are representative of two independent experiments.