Table 2.

Hybrid combination number, GUS activity, and multiplex PCR results.

Hybrid combination	GUS expression	Multiplex PCR			RT- PCR
		Gus	NptII	Bgt	Gus	NptII	Bgt
TP22	●	+	+	+	+	+	+
TP73×TP22 (287)	◐	+	+	+	+	+	+
TP23×TP49 (116)	●	+	+	+	+	+	+
TP23	●	+	+	+	+	+	+
WT×TP23 (212)	◐	+	+	+	+	+	+
TP73×WT (9)	○	+	+	–	S	S	S
WT×TP22 (184)	●	+	+	+	+	+	+
TP22×TP22 (284)	◐	+	+	+	+	+	+
TP23×TP49 (196)	○	+	+	+	s	+	+
TP23×WT (29)	◐	+	+	+	+	+	+
TP73	○	+	+	–	s	s	–

●Indicates GUS enzyme activity; ○indicates no GUS enzyme activity; ○ indicates partial GUS enzyme activity; “S” indicates gene silencing.