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L-citrulline pretreatment prevents high glucose-induced decrease in arginase activity in endothelial cells. NO production percentage in BAECs representing an average of three separate experiments done in six replicates. NO production decreased by 30% as compared to ionomycin stimulated control. Pretreating endothelial cells L-citrulline (2.5 mM), restored NO production to normal levels. Statistical analysis performed using one-way ANOVA followed by Tukey’s post hoc test *p < 0.05.
