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. 2020 Dec 22;11:618560. doi: 10.3389/fpls.2020.618560

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Copyright © 2020 Xiao, Zhang, Yu, Lu, Mei, Deng, Zhang, Chen, Chu, Tong and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analyses of OsRR genes in the root and shoot of the seedlings treated by different forms of CKs in the root. The roots of 10-day-old seedlings of OsPUP1-overexpressing plant (OE2) and the wild type (WT) were treated by mock solution, or different CK nucleobases at 0.01 μM concentrations for 4 h, and then the shoot and root tissues were separately collected for gene expression analyses. Ubiquitin2 gene was used as the internal reference. Genes and tissues are marked in each panel. n = 3, bar = SD. *P < 0.05 and **P < 0.01 in Student’s t-test. The values of WT shoots without CK treatment (Mock) were set to 1, and other values were the relative values compared to them. Asterisks indicate statistically significant difference of OE2, compared with WT. The pairs with statistically significant difference were calculated for the ratios of the relative expression of OE2 to WT.