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. Author manuscript; available in PMC: 2021 Jan 5.
Published in final edited form as: Mol Cell Neurosci. 2019 Nov 23;102:103449. doi: 10.1016/j.mcn.2019.103449

Fig. 7.

Fig. 7.

ITI-214 enhances ADP-induced p-VASP (S157) levels in cultured primary rat microglia. Microglia were treated with ITI-214 for 30 min prior to stimulation with ADP for 5 min. (A) Levels of phosphorylation of VASP at serine 157 were measured on a Western blot. Insets show representative samples; the same samples were loaded on each blot in the order indicated in the bar graphs. Green bands are p-VASP (top) or total VASP (bottom). Samples were normalized to actin as a loading control (red bands). Data was normalized to vehicle and statistical significance was determined with an ANOVA. Relative significance: **p < 0.01, ****p < 0.0001 compared to vehicle alone, #p < 0.05 compared to ADP alone.