Fig. 4.

. SCH58261 preserves the mitotic behaviour in NG2⁺ cells post-injury. The SHAM group received no treatment, the INJ group was exposed to 20 min of OGD, and the INJ+SCH58261 group was exposed to 20 min of OGD and received 1 μM SCH58261. (A) Immunofluorescent images of NG2⁺ cells (green areas). (B) Quantification of BrdU⁺nuclei/cells . (C) Co-stained cultures for BrdU (green areas) and NG2⁺ OPCs (refer to the white arrows pointing to the red areas). Scale bar: 50 µm. (D) Quantification of proliferating NG2⁺ cells. Data are shown as mean ± SEM (n = 6 per group). (*) indicates p < 0.05, (**) indicates p < 0.01, and (***) indicates p < 0.001.