Fig. 9.

Effect of DON on DNA damage of SH-SY5Y cells by comet assay for 24 h. (a) Dose-dependent effect of DON on DNA damage (Olive tail movement) of cells. (b) Depict the Olive tail movement (DNA damage) of cells exposed with different concentrations of DON at 0 (control), 25, 50, and 100 µM. The images were captured at a magnification of 400x. The studies were executed in six replicates (n = 6) and results were expressed as mean ± SD. The statistical difference between control and test groups was determined following Dunnett’s test and significance was determined at p ≤ 0.05 (* ≤ 0.05, ** < 0.01, and *** < 0.001). The p-value was considered as not significant at > 0.05 and denoted as ‘#’.