Extended Data Figure 4. Lack of detectable Aβ antibody titers in normal and Ctrl antibody-treated APP23 mice.

Plasma was taken for analysis from randomly selected 6-month-old male APP23 tg mice, either non-treated (tg, n=5) or treated for 5 consecutive days with Ctrl1 or Ctrl2 antibody and analyzed 6 weeks later (n=5/group; same mice as presented in Fig. 1). As a positive control, plasma from the Beta1- and cmAdu-injected mice one day after the injection were included (n=5 each) together with a pool of non-tg mice as a further negative control. Two different ELISA setups were used and performed on two consecutive days, one optimized to measure Beta1 titers (a) and another one optimized to measure cmAdu titers (b-d) (see Methods). Data are represented as group means ± SEMs. Results reveal that the injections of Ctrl antibodies into APP23 tg or non-tg mice did not induce Aβ antibody titers. Further, no detectable titers were found in untreated APP23 tg or non-tg mice.