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. 2020 Dec 1;10(12):4287–4307.

Figure 5.

Figure 5

Inhibition of caspase-associated inflammatory signals by vitamin D could effectively inhibit the occurrence of normal tissue cellular pyroptosis. (A) FCM revealed cisplatin-induced cell death in NOKs and GSEs after pre-treatment with aspirin, celecoxib, hydrocortisone, vitamin C and vitamin D; (B) Statistical analysis of the cell death rates shown in (A). Aspirin and vitamin D show the effect of inhibiting cell death during cisplatin treatment; (C) ELISA revealed reduced IL-1β secretion with cisplatin in NOKs and GSEs pre-treated vitamin D; (D) Gradient concentration of cisplatin-treated NOK and GSE pre-treated with vitamin D, which increased cisplatin tolerance; (E) Immunoblot analysis showing that vitamin D pre-treatment could inhibit the cleavage of caspase-1, caspase-3 and GSDME after cisplatin treatment; (F) Cytotoxicity assay revealed that LDH release reduced in NOKs and GSEs pre-treated with vitamin D compared with control cells after cisplatin treatment. (Vitamin D treatment: 30 nM, 3 day; cisplatin treatment: NOK 30 μM; GSE 20 μM, 24 hours for A, C, E, and F).