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. 2020 Dec 1;10(12):4325–4341.

Figure 4.

Figure 4

Knockdown of CDK6 suppresses the expression of MMP2 through transcriptional regulation. A. The quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the expression of MMP2 and MMP9 mRNAs in MDA-MB 231 and MDA-MB-231 sh-CDK6 pooling cells. Columns represent means of triplicate assays normalized to GAPDH (*P < 0.05). B. Protein expression levels of CDK6, c-Jun, Sp1, MMP-2, and MMP-9 in MDA-MB 231 and MDA-MB-231 sh-CDK6 pooling cells were determined by western blotting. C. Immunohistochemistry confirmed nuclear translocation of c-Jun and Sp1 in MDA-MB 231 and MDA-MB-231 sh-CDK6 pooling cells. D. A chromatin immunoprecipitation-qRT-PCR analysis was performed to determine the status of c-Jun and Sp1 in the MMP-2 gene promoter. Experiments were performed in triplicate (*P < 0.05). E. Representative immunohistochemical staining for c-Jun and Sp1, in tumors with high and low CDK6 expression. Original magnification: 40 ×, scale bar: 10 µm. F. Representative immunohistochemical staining for c-Jun and Sp1, in tumors with Ribociclib. Original magnification: 40 ×, scale bar: 10 µm.