Figure 5.

Effects of α₁-blockers on the migration of bladder cancer cells. Wound-healing assay in UMUC3 (A), 5637-AR (B), UMUC3-AR-shRNA (C), and 5637 (D) cells. The cells grown to confluence were gently scratched, and the wound area was measured after 24-hour culture in serum-free medium containing ethanol (mock), silodosin (10 µM), tamsulosin (1 µM), or naftopidil (10 µM). The migration determined by the rate of cells filling the wound area is presented relative to that in each line with mock treatment. Each value represents the mean (± SD) from three independent experiments. *P<0.001 (vs. mock treatment).