Effects of α1-blockers on the expression of ELK1/NF-κB in urothelial cells. Western blot of ELK1 and NF-κB in SVHUC-AR (A) and UMUC3 (B) cells treated with ethanol (mock), silodosin (Sil, 10 µM), tamsulosin (Tam, 1 µM), or naftopidil (Naf, 10 µM) for 48 hours. GAPDH served as an internal control. Densitometry values for ELK1/NF-κB standardized by GAPDH that are relative to those of mock treatment are included below the lanes.