Skip to main content
. Author manuscript; available in PMC: 2021 Jan 5.
Published in final edited form as: J Am Chem Soc. 2020 Dec 16;142(52):21679–21690. doi: 10.1021/jacs.0c07914

Figure 4.

Figure 4.

Comparison of 95Mo CW ENDOR acquired at the three major g-values for E4(4H) state trapped during Ar turnover of 95Mo enriched V70I, as reported47 and for E4(2H)* photogenerated from E4(4H) by irradiation with 450 nm laser diode at 12 K. “Goalposts” indicate 95Mo (I = 5/2) transition between the mI = ±1/2 nuclear sublevels with frequencies ν± = ∣ν(95MO) ± A/2∣. Most of the spectra show the higher-frequency (ν+) partner; the lower-frequency (ν−) partner is at such a low frequency that it is not reliably detected. Spectra from unenriched enzyme are featureless, as demonstrate by the one included from g3. Conditions; T = 2 K; microwave frequency, 35.1 and 34.8 GHz for E4(4H) and E4(2H)* correspondingly; microwave power, 1 μW and 0.1 μW; modulation amplitude, 4 and 1 G; RF sweep speed, 1 MHz/s and 2 MHz/s; time constant, 64 ms; RF bandwidth-broaden to 100 kHz.