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. 2021 Jan 5;40:6. doi: 10.1186/s13046-020-01791-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — CircLMO7 promotes GC malignant biological functions through glutamine metabolism. a. TCGA database analysis showed that GLS was highly expressed in GC tissues. b. qRT-PCR verified that GLS was highly expressed in GC tissues. c, d. qRT-PCR and Western blot analysis showed that circLMO7 and GLS were positively related. e-h. Overexpressing circLMO7 increased the expression levels of GLN, GLS, GLU and α-KG. Knocking down circLMO7 blocked this phenomenon. i, j. Colony formation and EdU assays showed that the promoting effect of ov-circLMO7 on GC cell proliferation was rescued after cotransfection with si-GLS; EdU scale bar = 25 μm. k. Transwell assays showed that si-GLS rescued the promoting effect of ov-circLMO7 on GC cell migration and invasion; scale bar = 100 μm. l. The active oxygen assay showed that the inhibitory effect of ov-circLMO7 on the production of reactive oxygen species in GC cells was rescued after cotransfection with si-GLS; scale bar = 50 μm. All data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001