Table 4.
Diagnostic utility of T-SPOT.TB (PF & PB) and ADA for diagnosing TPE
| Variables | Cut-off value a | Sensitivity% | Specificity% | PPV% | NPV% | +LR | -LR | DOR | AUC | 95%CI |
|---|---|---|---|---|---|---|---|---|---|---|
| Peripheral blood (PB) | ||||||||||
| ESAT-6 | 24# | 77.9 | 77.0 | 73.9 | 80.7 | 3.39 | 0.29 | 11.80 | ||
| 22¶ | 82.1 | 75.3 | 73.5 | 83.4 | 3.32 | 0.24 | 13.98 | 0.840 | 0.794–0.878 | |
| CFP-10 | 24# | 72.4 | 74.7 | 70.5 | 76.5 | 2.86 | 0.37 | 7.75 | ||
| 22¶ | 75.2 | 74.7 | 71.2 | 78.3 | 2.97 | 0.33 | 8.95 | 0.796 | 0.747–0.839 | |
| Max SFCs | 24# | 86.9 | 69.0 | 70.0 | 86.3 | 2.80 | 0.19 | 14.77 | ||
| 22¶ | 90.3 | 67.2 | 69.7 | 89.3 | 2.75 | 0.14 | 19.07 | 0.839 | 0.794–0.884 | |
| Pleural fluid (PF) | ||||||||||
| ESAT-6 | 170¶ | 86.9 | 78.2 | 76.8 | 87.7 | 3.99 | 0.17 | 23.80 | 0.874 | 0.833–0.909 |
| CFP-10 | 142¶ | 85.5 | 73.6 | 72.9 | 85.9 | 3.24 | 0.20 | 16.44 | 0.857 | 0.813–0.894 |
| Max SFCs | 466¶ | 81.4 | 82.8 | 79.7 | 84.2 | 4.73 | 0.22 | 21.07 | 0.885 | 0.846–0.923 |
| ADA | 22.4¶ | 89.0 | 83.9 | 82.2 | 90.1 | 5.53 | 0.13 | 42.16 | 0.887 | 0.847–0.920 |
| 40 | 71.0 | 93.1 | 89.6 | 79.4 | 10.29 | 0.31 | 33.03 | |||
ADA adenosine deaminase, TPE tuberculous pleural effusion, NPV negative predictive value, PPV positive predictive value, +LR positive likelihood ratio, −LR negative likelihood ratio, DOR diagnostic odds ratio, SFCs spot-forming cells, Max the larger of ESAT-6 and CFP-10, AUC the are under curve
#: manufacturer derived cut-off point for PB; ¶: AUC derived cut-off point
a Values are expressed as IU/L for ADA, and as SFCs/106 mononuclear cells for T-SPOT