Table 2.

Expected and observed relative abundance (%) of genera in mock community sample HM-783D. Setup 1 (2-step PCR; V3 V4 region); Setup 2 (2-step PCR; V4 region); Setup 3 (1-step PCR; V4 region)

Genera	Expected	Setup 1 (I)	Setup 1 (II)	Setup 1 (III)	Setup 1 (IV)	Setup 2	Setup 3
Escherichia	21.91	27.68	23.99	25.20	26.65	22.54	32.90
Rhodobacter	21.91	5.98	9.52	9.23	8.94	11.00	8.77
Staphylococcus	24.10	29.02	29.27	29.66	30.56	29.88	24.98
Streptococcus	24.12	28.51	27.39	27.03	25.38	26.20	25.81
Bacillus	2.19	3.38	2.86	2.95	2.85	3.15	2.49
Clostridium	2.19	2.18	3.28	2.19	1.88	2.64	1.69
Pseudomonas	2.19	1.44	1.68	1.75	1.93	2.12	2.02
Acinetobacter	0.22	0.32	0.29	0.33	0.30	0.29	0.12
Helicobacter	0.22	0.36	0.49	0.44	0.38	0.61	0.26
Lactobacillus	0.22	0.22	0.20	0.23	0.24	0.35	0.18
Listeria	0.22	0.33	0.33	0.30	0.32	0.37	0.26
Neisseria	0.22	0.24	0.31	0.30	0.27	0.43	0.39
Propionibacterium	0.22	0.13	0.22	0.18	0.15	0.29	0.00
Actinomyces	0.02	0.01	0.01	0.00	0.00	0.01	0.00
Bacteroides	0.02	0.02	0.00	0.03	0.02	0.04	0.02
Deinococcus	0.02	0.02	0.04	0.03	0.02	0.03	0.02
Enterococcus	0.02	0.03	0.02	0.03	0.02	0.02	0.00
Other	0.00	0.13	0.11	0.10	0.09	0.03	0.08