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Primers Used for q-RT-PCR Analysis.
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| ALP | TAAGGACATCGCCTACCAGC | TGGCTTTCTCGTCACTCTCA |
| OCN | TGAGAGCCCTCACACTCCTC | ACCTTTGCTGGACTCTGCAC |
| RUNX2 | CACCATGTCAGCAAAACTTCTT | TCACGTCGCTCATTTTGC |
| COLI | GTACTGGATTGACCCCAACC | ACCAGACATGCCTCTTGTCC |
| GAPDH | GGCTCTCCAGAACATCATCC | TTTCTAGACGGCAGGTCAGG |
ALP: alkaline phosphatase; COL1: collagen type I; OCN: osteocalcin; q-RT-PCR: quantitative real-time PCR; RUNX2: runt-related transcription factor 2.
