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. 2020 Nov 10;29:0963689720967672. doi: 10.1177/0963689720967672

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 1. — Effect of GLA on cell viability of H9c2 cells. (A) LDH leakage assay was performed to assess the cytotoxic effect of GLA on H9c2 cells after incubation with a series concentration of GLA (0, 5, 10, 20, and 40 μM) for 24 h. (B) MTT assay was performed to assess the protective effect of GLA on H/R-stimulated H9c2 cells. H9c2 cells were preincubated with GLA (5, 10, and 20 μM) for 2 h, and then subjected to H/R stimulation. *P < 0.05 indicates the significant difference compared with control H9c2 cells. ^# P < 0.05 indicates the significant difference compared with H/R-stimulated H9c2 cells. GLA: glaucocalyxin A; H/R: hypoxia/reoxygenation; LDH: lactate dehydrogenase; MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.