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. 2020 Nov 10;29:0963689720967672. doi: 10.1177/0963689720967672

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 3. — Effect of GLA on apoptosis in H9c2 cells. H9c2 cells were preincubated with GLA (5, 10, and 20 μM) for 2 h, and then subjected to H/R stimulation. (A) Western blot analysis was performed to detect the expression levels of apoptosis-related proteins, including bax and bcl-2. (B, C) Quantification analysis of bax and bcl-2. (D) Colorimetric method was used to determine the caspase-3 activity with the substrate peptide Ac-DEVD-pNA. *P < 0.05 indicates the significant difference compared with control H9c2 cells. ^# P < 0.05 indicates the significant difference compared with H/R-stimulated H9c2 cells. GLA: glaucocalyxin A; H/R: hypoxia/reoxygenation.