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. 2020 Nov 10;29:0963689720967672. doi: 10.1177/0963689720967672

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 4. — Effect of GLA on the Akt/Nrf2/HO-1 signaling pathway in H9c2 cells. H9c2 cells were preincubated with GLA (5, 10, and 20 μM) for 2 h, and then subjected to H/R stimulation. (A) Western blot analysis was performed to detect the expression levels of p-Akt, Akt, nuclear Nrf2, and HO-1. (B) The ratio of p-Akt/Akt. (C) The ratio of nuclear Nrf2/lamin B1. (D) The ratio of HO-1/β-actin. ^# P < 0.05 indicates the significant difference compared with H/R-stimulated H9c2 cells. Akt: protein kinase B; GLA: glaucocalyxin A; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2.