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. 2020 Nov 10;29:0963689720967672. doi: 10.1177/0963689720967672

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 5. — Effect of LY294002 on cell viability, oxidative stress, and apoptosis in H9c2 cells. H9c2 cells were preincubated with GLA (20 μM) and/or LY294002 (10 μM) for 2 h and then subjected to H/R stimulation. (A) MTT assay was performed to assess cell viability. (B) Flow cytometry was performed to evaluate ROS production. (C) Colorimetric method was used to determine the caspase-3 activity. *P < 0.05. GLA: glaucocalyxin A; H/R: hypoxia/reoxygenation; MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; ROS: reactive oxygen species.