Figure 5.

Apoptosis is reduced by local pretreatment of islets with liraglutide as compared to systemic injection of liraglutide in STZ-diabetic mice transplanted with islet. (A–D) TUNEL (red), insulin (green), 4′-6-diamidino-2-phenylindole (blue) and hematoxylin/eosin (H&E) staining of kidney section. Representative images are shown (A) I300, (B) I200, (C) I200-local, and (D) I200-systemic groups on 35 days after islet transplantation (Scale bars, 10 μm). White arrows indicate TUNEL⁺insulin⁺ cells. (E) Quantification of TUNEL⁺insulin⁺ apoptotic beta cells in the transplanted islets. Percentile of apoptotic β cells was calculated as the number of TUNEL⁺insulin⁺ cells divided by the number of insulin⁺ cells in islet grafts; n =3 mice/group. The data are presented as mean ± SD, *P < 0.05, **P < 0.01. STZ: streptozotocin; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling.