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. 2020 Oct 27;29:0963689720929683. doi: 10.1177/0963689720929683

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Culture of crypts from mouse colon with MSC improves organoid formation. (A) Pictures of crypts from colon on the day of seeding. The number of crypts was counted in each culture well (upper left panel) ×4 magnification. The day after, buds visualized in 3D were counted (upper right panel). Colonoids (lower left panel) and spheroids (lower right panel) were formed over time. ×10 magnification (B) Graph representing the number of organoids with or without co-culture with MSC in different culture conditions (normal concentration of growth factors and half concentration). Normalization according to the number of seeded crypts at day 0 was applied. Experiments were performed in triplicate with 3 different preparations of MSC. Results are expressed as mean ± SEM.