Figure 6.

MSC co-culture improves organoid formation. (A) Pictures of organoids from GFP mice (left panels) cultivated with or without MSC in half growth factor concentration. Proliferation of cells was visualized using Ki-67 immunostaining (right panels). (B) Pictures of organoids fixed in formaldehyde at 5 days of culture at lower and greater magnifications (in the middle small squares) to allow size measurement by computational counting. (C) Graph representing the size of organoids (µm²) when crypts were cultured in the presence of MSC or not. These results were obtained in 2 separate experiments performed in duplicate. Results are expressed as mean ± SEM