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. 2020 Oct 7;29:0963689720965182. doi: 10.1177/0963689720965182

Figure 2.

Figure 2.

GASAL1 promotes cell proliferation and G1-to-S phase progression of HTR-8/SVneo and JAR cell lines. Cells were transfected with GASAL1 siRNA (10 nM or 30 nM) and pcDNA GASAL1 (0.5 μg/ml or 2 μg/ml). (A and C) The efficiencies of GASAL1 siRNA and pcDNA GASAL1 were determined by qPCR. (B and D) Cell proliferation was detected by cell counting kit-8 assay at 0 h, 24 h, 48 h, 72 h, and 96 h in HTR-8/SVneo and JAR cell lines. (E–H). Cell cycle was analyzed by flow cytometry after 24 h of cell transfection with GASAL1 siRNA or pcDNA GASAL1 in HTR-8/SVneo (E and F) and JAR (G and H) cell lines. The data were presented as the mean ± SEM, n = 3. Student’s t-test or one-way analysis of variance was used for comparisons between groups in this study. *P < 0.05 and **P < 0.01. GASAL1: lncRNA growth arrest associated lncRNA 1; lncRNA: long noncoding RNA; pcDNA: plasmid cloning DNA; qPCR: quantitative polymerase chain reaction; siRNA: short interfering RNA.