Figure 3.

GASAL1 inhibits cell apoptosis and promotes cell invasion in HTR-8/SVneo and JAR cell lines. Cells were transfected with GASAL1 siRNA (10 nM or 30 nM) and pcDNA GASAL1 (0.5 μg/ml or 2 μg/ml). (A and D) Cell apoptosis was detected by flow cytometry after cell transfection for 24 h in HTR-8/SVneo and JAR cell lines. (B and E) Expression of apoptosis-related protein (cleaved-caspase-3 and Bcl-2) were determined by Western blotting in HTR-8/SVneo and JAR cell lines. (C and F) Transwell assay was used to analyze cell invasion in HTR-8/SVneo and JAR cell lines. The data were presented as the mean ± SEM, n = 3. Student’s t-test was used for comparisons between groups in this study. *P < 0.05 and **P < 0.01. GASAL1: lncRNA growth arrest associated lncRNA 1; lncRNA: long noncoding RNA; pcDNA: plasmid cloning DNA; siRNA: short interfering RNA.