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. 2020 Oct 12;29:0963689720962052. doi: 10.1177/0963689720962052

Figure 9.

Figure 9.

Effect of Nrf2 knockdown on CTRP9-mediated retinal-protective effect. ARPE-19 cells were cotransfected with pcDNA3.0-CTRP9 and si-Nrf2 for 48 h, then subjected to HG stimulation. (A) Western blot was used to assess Nrf2 expression. (B) Cell viability was detected by CCK-8 assay. (C–E) Detection of ROS production, MDA content, and caspase-3 activity. *Significantly different compared to control group. #Significantly different compared to HG group. &Significantly different compared to HG + CTRP9 group. CCK-8: cell counting kit-8; CTRP9: C1q/TNF-related protein 9; HG: high glucose; MDA: malondialdehyde; ROS: reactive oxygen species.