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. 2020 Sep 21;29:0963689720960174. doi: 10.1177/0963689720960174

Fig. 5.

Fig. 5.

Circ_002178 facilitated KDM7A expression through miR-1258. (A) The targeted binding of miR-1258 to KDM7A was predicted by TargetScan. (B and C) SUM159PT cells were transfected with NC inh or miR-1258 inh, while MDA-MB-231 cells were transfected with miR-NC or miR-1258. (B) The target relationship was confirmed by double luciferase report analysis (**P < 0.01 vs NC inh or miR-NC). (C) The expression of KDM7A was measured by Western blotting (**P < 0.01 vs NC inh or miR-NC). (D) The expression of KDM7A in cancer tissues and paracarcinoma tissues was measured by Western blotting. (E) The expression of KDM7A in cancer tissues and paracarcinoma tissues was measured by RT-qPCR (**P < 0.01 vs normal). (F) The expression correlation of hsa_circ_002178 and KDM7A or miR-1258 and KDM7A was analyzed using Pearson’s correlation analysis. (G) SUM159PT cells were transfected with miR-NC or/and miR-1258, while MDA-MB-231 cells were transfected with shRNA2# or/ and miR-1258 inh (**P < 0.01 vs mock + miR-NC or circRNA + miR-NC or shNC + NC inh or shRNA2# + NC inh). circRNA: circular RNA; NC inh: negative control inhibitor; RT-qPCR: quantitative reverse transcription PCR.