TABLE 2.

Primers used for realtime-PCR, methylation-specific PCR and bisulfite- sequencing PCR.

Method	Primers	Sequence	Length (bp)	Temp (°C)
Realtime-PCR	HHIP	5′-CTGCTTCTGTATTCAGGAGGTT-3′	229	55
		5′-GGGATGGAATGCGAGGCTTA-3′
	β-actin	5′-AGAGCTACGAGCTGCCTGAC-3′	184	60
		5′-AGCACTGTGTTGGCGTACAG-3′
BSP	HHIP	5′-GGGGAGGAGAGAGGAGTTTG-3′	243	60
		5′-CCCRACRACCTCCCTACTAC-3′
	β-actin	5′-AGAGCTACGAGCTGCCTGAC-3′	184	60
		5′-AGCACTGTGTTGGCGTACAG-3′
MSP	Methylation	5′-GTAGTAGTCGGGTAGTTTCGGAATTTTC-3′	190	60
		5′-AAAAACGACTAACCGCGACG-3′
	Non-methylation	5′-AGTAGTTGGGTAGTTTTGGAATTTTTGG-3′	188	60
		5′-AAAAACAACTAACCACAACA-3′

HHIP, hedgehog-interacting protein; BSP, bisulfite-sequencing PCR; and MSP, methylation-specific PCR.