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. Author manuscript; available in PMC: 2021 Jan 5.
Published in final edited form as: Geosciences (Basel). 2019;10(1):5. doi: 10.3390/geosciences10010005

Table 1.

Colony forming units spreadplate (CFU-S) assay data. Total colony forming units (CFU) recovered (% recovery) from all inoculated plates (n = 10).

Soils Total CFU/10 Plates (Relative Percent Recovery)

(Number of Spiked Endospores/9 Grams Soil)

0 4.5 45 225 675 1350
Loam 1 0 0 2 (55.6) 10 (54.9) 28 (51.9) 72 (66.7)
Loam 2 0 2 (556) 1 (27.8) 9 (49.5) 14 (25.9) 61 (56.5)
Loam 3 0 1 (278) 0 12(66.7) 32 (59.3) 82 (75.9)
Sand 1 0 0 0 16 (89.9) 20 (37.1) 42 (38.9)
Sand 2 0 0 2 (55.6) 13 (71.4) 47 (87.0) 80 (74.1)
Sand 3 0 0 0 3 (16.7) 51 (90.7) 66 (59.3)
Clay 1 0 0 6 (166) 25 (138) 91 (169) 178 (165)
Clay 2 0 0 5 (139) 22 (121) 673 (>500) 176 (163)
Clay 3 0 1 (278) 5 (139) 24 (132) 59 (109) 159 (147)

Expected recovery totals using 10 spread plates of 200 ul for each spike level for all soil types—0 = 0, 4.5 = 0.36 total CFU, 45 = 3.6 total CFU, 225 = 18.2 total CFU, 675 = 54.0 total CFU, 1350 = 108.0.