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. Author manuscript; available in PMC: 2021 Jan 5.
Published in final edited form as: Geosciences (Basel). 2019;10(1):5. doi: 10.3390/geosciences10010005

Table 4.

Colony-forming unit spreadplate (CFU-S) data versus liquid culture enrichment-polymerase chain reaction (E-PCR) data.

Soils CFU vs. PCR
(Number of Spiked Endospores/9 Grams Soil)
0 (CFU/PCR) 4.5 (CFU/PCR) 45 (CFU/PCR) 225 (CFU/PCR) 675 (CFU/PCR) 1350 (CFU/PCR)
Loam 1 −/− −/− −/+ +/+ +/+ +/+
Loam 2 −/− −/+ −/+ −/+ +/+ +/+
Loam 3 −/− −/+ −/+ +/+ +/+ +/+
Sand 1 −/− −/+ −/+ +/+ +/+ +/+
Sand 2 −/− −/+ −/+ +/+ +/+ +/+
Sand 3 −/− −/+ −/+ −/+ +/+ +/+
Clay 1 −/− −/+ −/+ +/+ +/+ +/+
Clay 2 −/− −/+ −/+ +/+ +/+ +/+
Clay 3 −/− −/+ −/+ +/+ +/+ +/+

+ equals sample positive - CFU average of ≥1 CFU for the 10-plate sample set or PCR positive. – equals sample negative - CFU average of <1 CFU for the 10-plate sample set or PCR negative.