FIGURE 3.

¹⁵N‐HSQC titration data for BRD3 and BRD4 BD‐H2A.Z interactions. (a–d) ¹⁵N‐HSQC titrations monitoring the addition of the indicated H2A.Z peptides (up to 1.1 mM peptide—~10 molar equivalents) to BRD3‐BD1 (a), BRD3‐BD2 (b), BRD4‐BD1 (c), and BRD4‐BD2 (d). The concentrations of BDs in each experiment are ~100 μM. (e‐h) Binding curves derived by tracking the ¹⁵NCSPs induced in BRD3‐BD1 I114 (e), BRD3‐BD2 D339 (f), BRD4‐BD1 I138 (g), and BRD4‐BD2 D381 (h), in response to titration with the indicated H2A.Z peptide variants. The data were fitted to a simple 1:1 binding isotherm using GraphPad