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. 2020 Jul 24;14(12):2967–2979. doi: 10.1038/s41396-020-0724-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Sequences obtained in this study are printed in bold. “Ca. N. alkalitolerans” is the Nitrospira species cultured and further analyzed in this study. The tree was constructed using full length sequences and a 50% conservation filter resulting in 1310 valid alignment positions. Shorter sequences from this study, generated through amplicon and Sanger sequencing were added to the tree using the Evolutionary Placement Algorithm (EPA) without changing the overall tree topology. Numbers in brackets behind these sequences firstly denote the likelihood score of the exact placement and secondly the cumulative likelihood score of the placement within the cluster. Filled, gray, and open circles denote branches with ≥90%, ≥70% and ≥50% bootstrap support, respectively. Leptospirillum ferrooxidans (AJ237903), Ca. Magnetobacterium bavaricum (FP929063), Thermodesulfovibrio yellowstonii DSM 11347 (CP001147), and Ca. Methylomirabilis oxyfera (FP565575) were used as outgroup. The scale bar indicates 6% estimated sequence divergence.